Abstract
COS 1 cells were transfected with full-length pig stomach sarcoplasmic/endoplasmic reticulum Ca2+ pump (SERCA)2a or SERCA2b cDNA. Ca2+ uptake by microsomes from transfected cells revealed that the Ca2+ affinity of the SERCA2b Ca2+ pump (K0.5 0.17 +/- 0.01 microM) was higher than that of the SERCA2a Ca2+ pump (K0.5 0.31 +/- 0.02 microM). Thapsigargin-sensitivity was found to be identical for the two isoforms. The Ca2+ affinity of both the SERCA2a and SERCA2b Ca2+ pumps was decreased by a factor of two when they were co-expressed with phospholamban.
MeSH terms
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Animals
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Biological Transport
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Blotting, Western
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Calcium / metabolism
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Calcium-Binding Proteins / pharmacology*
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Calcium-Transporting ATPases / drug effects
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Calcium-Transporting ATPases / genetics
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Calcium-Transporting ATPases / metabolism*
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Calcium-Transporting ATPases / physiology*
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Cell Line
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DNA
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Electrophoresis, Polyacrylamide Gel
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Endoplasmic Reticulum / metabolism
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Gastric Mucosa / metabolism
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Immunohistochemistry
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Microsomes / metabolism
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Sarcoplasmic Reticulum / metabolism
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Swine
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Terpenes / pharmacology
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Thapsigargin
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Transfection
Substances
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Calcium-Binding Proteins
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Terpenes
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phospholamban
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Thapsigargin
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DNA
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Calcium-Transporting ATPases
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Calcium