Cloning of a delta opioid receptor by functional expression

Science. 1992 Dec 18;258(5090):1952-5. doi: 10.1126/science.1335167.

Abstract

Opiate drugs have potent analgesic and addictive properties. These drugs interact with receptors that also mediate the response to endogenous opioid peptide ligands. However, the receptors for opioids have eluded definitive molecular characterization. By transient expression in COS cells and screening with an iodinated analog of the opioid peptide enkephalin, a complementary DNA clone encoding a functional delta opioid receptor has been identified. The sequence shows homology to G protein-coupled receptors, in particular the receptors for somatostatin, angiotensin, and interleukin-8.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Binding, Competitive
  • Blotting, Northern
  • Blotting, Southern
  • Cell Line
  • Cyclic AMP / metabolism
  • Diprenorphine / metabolism
  • Enkephalin, D-Penicillamine (2,5)-
  • Enkephalins / pharmacology
  • Etorphine / pharmacology
  • Gene Expression
  • Humans
  • Kinetics
  • Models, Structural
  • Molecular Sequence Data
  • Naloxone / pharmacology
  • Narcotics / pharmacology
  • Protein Structure, Secondary
  • Receptors, Opioid, delta / chemistry
  • Receptors, Opioid, delta / genetics*
  • Receptors, Opioid, delta / metabolism*
  • Sequence Homology, Amino Acid
  • Transfection
  • Tumor Cells, Cultured

Substances

  • Enkephalins
  • Narcotics
  • Receptors, Opioid, delta
  • Diprenorphine
  • Naloxone
  • Etorphine
  • Enkephalin, D-Penicillamine (2,5)-
  • Cyclic AMP