Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression

Nat Genet. 1992 Nov;2(3):173-9. doi: 10.1038/ng1192-173.

Abstract

Large scale sequencing of cDNAs provides a complementary approach to structural analysis of the human genome by generating expressed sequence tags (ESTs). We have initiated the large-scale sequencing of a 3'-directed cDNA library from the human liver cell line HepG2, that is a non-biased representation of the mRNA population. 982 random cDNA clones were sequenced yielding more than 270 kilobases. A significant portion of the identified genes encoded secretable proteins and components for protein-synthesis. The abundance of cDNA species varied from 2.2% to less than 0.004%. Fifty two percent of the mRNA were abundant species consisting of 173 genes and the rest were non-abundant, consisting of about 6,600 genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / genetics*
  • Gene Expression*
  • Gene Frequency
  • Gene Library*
  • Genome, Human*
  • Humans
  • Liver / chemistry*
  • Liver / cytology
  • Molecular Sequence Data
  • Proteins / genetics
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Tumor Cells, Cultured

Substances

  • DNA, Complementary
  • Proteins
  • RNA, Messenger