Skeletal muscle and brain isoforms of a beta-subunit of human voltage-dependent calcium channels are encoded by a single gene

J Biol Chem. 1992 Nov 15;267(32):22967-72.

Abstract

Clones of the beta 1-subunit of the voltage-dependent calcium channel (VDCC) from human skeletal muscle and hippocampus cDNA libraries, and from human genomic libraries, were isolated using a human skeletal muscle beta 1 cDNA probe generated by polymerase chain reaction. The skeletal muscle beta 1 cDNA (beta 1M) encodes a protein of 523 amino acids that is 97% identical to the rabbit skeletal muscle beta-subunit. Two different cDNAs, beta 1B1 and beta 1B2, were obtained from the human hippocampus library. The beta 1B1 transcript encodes a protein of 478 amino acids that is identical to the skeletal muscle beta-subunit (beta 1M), except for an internal region of 52 amino acids. The beta 1B2 transcript encodes a protein of 596 amino acids. The beta 1B2 polypeptide is identical to the beta 1B1 polypeptide at amino acids 1-444; however, it has a unique 152 amino acid carboxyl terminus. Like beta 1B1, it differs from beta 1M at the internal 52 amino acids. Analysis of the beta 1 gene structure demonstrates that these three cDNAs represent transcripts encoded by a single beta 1 gene. Transcripts from the beta 1 gene were detected in RNA from skeletal muscle, heart, spleen, and brain, but not in RNA from liver, stomach, or kidney.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Calcium Channels / genetics*
  • Cloning, Molecular
  • Genes
  • Genomic Library
  • Hippocampus / physiology*
  • Humans
  • Macromolecular Substances
  • Molecular Sequence Data
  • Muscles / physiology*
  • Oligodeoxyribonucleotides
  • Organ Specificity
  • Polymerase Chain Reaction
  • RNA / genetics
  • RNA / isolation & purification
  • RNA Splicing
  • Rats
  • Sequence Homology, Amino Acid
  • Transcription, Genetic

Substances

  • Calcium Channels
  • Macromolecular Substances
  • Oligodeoxyribonucleotides
  • RNA

Associated data

  • GENBANK/D12748
  • GENBANK/D12749
  • GENBANK/D12750
  • GENBANK/D12751
  • GENBANK/D12752
  • GENBANK/D12753
  • GENBANK/M92300
  • GENBANK/M92301
  • GENBANK/M92302
  • GENBANK/M92303