Abstract
The monoclonal antibody OVTL3 has a highly restricted reactivity with ovarian carcinomas and defines a surface glycoprotein, OA3, which has been used for immunotargeting. To understand why OA3 is found on ovarian tumors we isolated a complementary DNA by expression cloning. The clone encodes a 323-amino acid protein with 5 putative membrane spanning domains, reminiscent of a membrane receptor or channel, but of a new type with little or no sequence similarity with these families of proteins. Interestingly, the OA3 sequence is highly related to a vaccinia virus encoded protein (VA38) and its extracellular domain is a member of the immunoglobulin V region superfamily.
MeSH terms
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Amino Acid Sequence
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Antigens, Neoplasm / genetics*
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Base Sequence
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Biomarkers, Tumor / genetics*
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Blotting, Southern
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Cloning, Molecular
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DNA, Neoplasm / genetics
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DNA, Neoplasm / isolation & purification
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DNA, Recombinant / genetics
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Female
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Genetic Variation / genetics
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Humans
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Immunoglobulin Variable Region / genetics*
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Membrane Proteins / genetics*
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Molecular Sequence Data
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Ovarian Neoplasms / genetics*
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Ovarian Neoplasms / immunology
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RNA Splicing
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RNA, Messenger / genetics
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Sequence Homology
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Tumor Cells, Cultured
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Vaccinia virus / genetics*
Substances
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Antigens, Neoplasm
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Biomarkers, Tumor
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DNA, Neoplasm
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DNA, Recombinant
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Immunoglobulin Variable Region
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Membrane Proteins
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RNA, Messenger
Associated data
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GENBANK/S44779
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GENBANK/S44781
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GENBANK/S44786
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GENBANK/S44787
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GENBANK/S72766
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GENBANK/S72767
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GENBANK/S72768
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GENBANK/S72769
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GENBANK/S72771
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GENBANK/X63098