Crystallization and preliminary X-ray study of the cathepsin B complexed with CA074, a selective inhibitor

A Yamamoto; T Kaji; K Tomoo; T Ishida; M Inoue; M Murata; K Kitamura

doi:10.1016/0022-2836(92)90234-b

Crystallization and preliminary X-ray study of the cathepsin B complexed with CA074, a selective inhibitor

J Mol Biol. 1992 Oct 5;227(3):942-4. doi: 10.1016/0022-2836(92)90234-b.

Authors

A Yamamoto¹, T Kaji, K Tomoo, T Ishida, M Inoue, M Murata, K Kitamura

Affiliation

¹ Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences, Japan.

PMID: 1404397
DOI: 10.1016/0022-2836(92)90234-b

Abstract

Cathepsin B from bovine spleen has been purified and crystallized as a complex with a specific inhibitor CA074 [N-(L-3-trans-propylcarbamoyloxirane-2-carbonyl)-L- isoleucyl-L-proline], using the hanging-drop method. The complex crystals obtained from 50 mM-citrate buffer (pH 3.5) belong to the tetragonal space group P4(1) (or P4(3)) with a = 73.06 A and c = 141.59 A, and diffract beyond 2.2 A resolution. There are two complex molecules per asymmetric unit giving a packing density of 3.37 A3/Da and indicating a high solvent content of 63.5%.

MeSH terms

Animals
Cathepsin B / chemistry*
Cathepsin B / metabolism
Cattle
Crystallization
Dipeptides / chemistry*
Dipeptides / metabolism
Macromolecular Substances
Models, Chemical
X-Ray Diffraction

Substances

Dipeptides
Macromolecular Substances
N-(3-propylcarbamoyloxirane-2-carbonyl)-isoleucyl-proline
Cathepsin B