Abstract
In order to generate marked insertions in the myxobacterium Sorangium cellulosum, a transposon based on the eukaryotic mariner transposon was developed. The transposition frequency was increased with the use of a mutated tnp gene. The transposon randomly inserts into the chromosome, as demonstrated by targeted mutagenesis of the epoK gene.
MeSH terms
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Bacterial Proteins / genetics
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Cytochrome P-450 Enzyme System / genetics
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DNA Transposable Elements*
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DNA-Binding Proteins / genetics*
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Mutagenesis, Insertional*
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Myxococcales / genetics*
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Oxidoreductases / genetics
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Transposases
Substances
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Bacterial Proteins
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DNA Transposable Elements
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DNA-Binding Proteins
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mariner transposases
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Cytochrome P-450 Enzyme System
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EpoK protein, Polyangium cellulosum
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Oxidoreductases
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Transposases