Single step isolation of sildenafil from commercially available Viagra tablets

Int J Impot Res. 2003 Oct;15(5):369-72. doi: 10.1038/sj.ijir.3901040.

Abstract

Sildenafil, the active ingredient in Viagra, has been purified from commercially available tablets. The purification, using Sephadex G25 chromatography under conditions of low ionic strength, is simple and inexpensive. Sildenafil purified according to this protocol has been characterized with respect to its IC50 for PDE5, its ultraviolet absorption profile, and by collision-induced dissociation fingerprinting, positive ion nanospray, and MALDI mass spectrometry. Tritated sildenafil (6 Ci/mmol) was prepared commercially using the sildenafil purified by this protocol and was verified to retain the potency of unlabeled sildenafil. This protocol and similar procedures will allow investigators to easily isolate sufficient amounts of sildenafil or other PDE5 inhibitors for conducting biochemical and in vitro studies of drug action.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Dextrans
  • Hydrophobic and Hydrophilic Interactions
  • Phosphodiesterase Inhibitors / chemistry
  • Phosphodiesterase Inhibitors / isolation & purification*
  • Piperazines / chemistry*
  • Piperazines / isolation & purification*
  • Purines
  • Sildenafil Citrate
  • Sulfones
  • Tritium

Substances

  • Dextrans
  • Phosphodiesterase Inhibitors
  • Piperazines
  • Purines
  • Sulfones
  • Tritium
  • sephadex
  • Sildenafil Citrate