Wild-type p53 binds to the TATA-binding protein and represses transcription

Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):12028-32. doi: 10.1073/pnas.89.24.12028.

Abstract

p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Nucleus / metabolism
  • Cells, Cultured
  • DNA-Binding Proteins / metabolism*
  • Fungal Proteins / metabolism
  • Gene Expression Regulation*
  • Genes, Tumor Suppressor
  • Humans
  • In Vitro Techniques
  • Protein Binding
  • Recombinant Proteins / metabolism
  • Species Specificity
  • Structure-Activity Relationship
  • TATA Box*
  • TATA-Box Binding Protein
  • Transcription Factors / metabolism*
  • Transcription, Genetic*
  • Tumor Suppressor Protein p53 / metabolism*

Substances

  • DNA-Binding Proteins
  • Fungal Proteins
  • Recombinant Proteins
  • TATA-Box Binding Protein
  • Transcription Factors
  • Tumor Suppressor Protein p53