Abstract
The molecular mechanism underlying the retention of intron-containing mRNAs in the nucleus is not understood. Here, we show that retention of intron-containing mRNAs in yeast is mediated by perinuclearly located Mlp1. Deletion of MLP1 impairs retention while having no effect on mRNA splicing. The Mlp1-dependent leakage of intron-containing RNAs is increased in presence of ts-prp18 delta, a splicing mutant. When overall pre-mRNA levels are increased by deletion of RRP6, a nuclear exosome component, MLP1 deletion augments leakage of only the intron-containing portion of mRNAs. Our data suggest, moreover, that Mlp1-dependent retention is mediated via the 5' splice site. Intriguingly, we found Mlp-proteins to be present only on sections of the NE adjacent to chromatin. We propose that at this confined site the perinuclear Mlp1 implements a quality control step prior to export, physically retaining faulty pre-mRNAs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Active Transport, Cell Nucleus / genetics
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Cell Nucleus / genetics*
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Cell Nucleus / metabolism
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Exoribonucleases / deficiency
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Exoribonucleases / genetics
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Exosome Multienzyme Ribonuclease Complex
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Introns / genetics
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Mutation / genetics
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Nuclear Envelope / genetics
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Nuclear Envelope / metabolism
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Nuclear Pore Complex Proteins / genetics
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Nuclear Pore Complex Proteins / metabolism
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Nuclear Proteins / deficiency
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Nuclear Proteins / genetics*
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Nuclear Proteins / metabolism
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Protein Transport / genetics
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RNA Splice Sites / genetics
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RNA Splicing / genetics*
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RNA, Messenger / genetics*
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RNA, Messenger / metabolism
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RNA-Binding Proteins
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Ribonucleoprotein, U5 Small Nuclear
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Saccharomyces cerevisiae Proteins / genetics*
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Saccharomyces cerevisiae Proteins / metabolism
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Yeasts / genetics*
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Yeasts / metabolism
Substances
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MLP1 protein, S cerevisiae
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Nuclear Pore Complex Proteins
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Nuclear Proteins
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PRP18 protein, S cerevisiae
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RNA Splice Sites
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RNA, Messenger
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RNA-Binding Proteins
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Ribonucleoprotein, U5 Small Nuclear
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Saccharomyces cerevisiae Proteins
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Exoribonucleases
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Exosome Multienzyme Ribonuclease Complex
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RRP6 protein, S cerevisiae