Characterization of psychrotrophic bacteria in the surface and deep-sea waters from the northwestern Pacific Ocean based on 16S ribosomal DNA analysis

O K Radjasa; H Urakawa; K Kita-Tsukamoto; K Ohwada

doi:10.1007/s10126-001-0050-1

Characterization of psychrotrophic bacteria in the surface and deep-sea waters from the northwestern Pacific Ocean based on 16S ribosomal DNA analysis

Mar Biotechnol (NY). 2001 Sep;3(5):454-62. doi: 10.1007/s10126-001-0050-1.

Authors

O K Radjasa¹, H Urakawa, K Kita-Tsukamoto, K Ohwada

Affiliation

¹ Marine Microbiology Division, Ocean Research Institute, University of Tokyo, 1-15-1 Minamidai, Nakano-ku, Tokyo 164-8639, Japan.

PMID: 14961338
DOI: 10.1007/s10126-001-0050-1

Abstract

Seventy-eight 4 degrees C-culturable bacteria were isolated using ZoBell 2216E medium from surface (0-200 m) and deep-sea (1000-9671 m) waters in the northwestern Pacific Ocean. Growth studies indicated that all 4 degrees C-culturable bacteria were psychrotrophs. Six phylotypes were observed in the surface water samples and 8 phylotypes in the deep-sea waters. Phylogenetic characterization based on 16S ribosomal DNA sequence analysis of the representative phylotypes revealed that some bacterial genera, Pseudoalteromonas, Photobacterium, and Vibrio, were common to surface and deep-sea waters, and others, Pseudomonas and Halomonas, specifically occurred in surface water. Overall, the members of Vibrionaceae appear to be dominant in both habitats.