-1 frameshifting at a CGA AAG hexanucleotide site is required for transposition of insertion sequence IS1222

J Bacteriol. 2004 May;186(10):3274-7. doi: 10.1128/JB.186.10.3274-3277.2004.

Abstract

The discovery of programmed -1 frameshifting at the hexanucleotide shift site CGA_AAG, in addition to the classical X_XXY_YYZ heptanucleotide shift sequences, prompted a search for instances among eubacterial insertion sequence elements. IS1222 has a CGA_AAG shift site. A genetic analysis revealed that frameshifting at this site is required for transposition.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • DNA Transposable Elements*
  • Frameshifting, Ribosomal*
  • Molecular Sequence Data
  • Rahnella / genetics*

Substances

  • DNA Transposable Elements

Associated data

  • GENBANK/AY528232