Abstract
Bioassay-directed fractionation of a butanone extract of Monochaetum vulcanicum resulted in the isolation of a new triterpene (1) and four known compounds, ursolic acid (2), 2alpha-hydroxyursolic acid (3), 3-(p-coumaroyl)ursolic acid (4), and beta-sitosteryl-beta-d-galactoside (5). The structure of the new compound 1 was established as 3beta-acetoxy-2alpha-hydroxyurs-12-en-28-oic acid on the basis of extensive 1D and 2D NMR spectroscopic interpretation and chemical derivatization. Compounds 1-3 and 5 exhibited polymerase beta lyase activity.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetylation
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Costa Rica
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DNA Polymerase beta / antagonists & inhibitors*
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Enzyme Inhibitors / chemistry
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Enzyme Inhibitors / isolation & purification*
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Enzyme Inhibitors / pharmacology
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Inhibitory Concentration 50
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Lyases / antagonists & inhibitors*
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Melastomataceae / chemistry*
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Molecular Structure
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Nuclear Magnetic Resonance, Biomolecular
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Plants, Medicinal / chemistry*
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Stereoisomerism
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Triterpenes / chemistry
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Triterpenes / isolation & purification*
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Triterpenes / pharmacology
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Ursolic Acid
Substances
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3beta-acetoxy-2alpha-hydroxyurs-12-ene-28-oic acid
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Enzyme Inhibitors
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Triterpenes
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DNA Polymerase beta
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Lyases