1-Hydroxy monocyclic carotenoid 3,4-dehydrogenase from a marine bacterium that produces myxol

FEBS Lett. 2004 Jul 16;570(1-3):184-8. doi: 10.1016/j.febslet.2004.05.085.

Abstract

A crtD (1-HO carotenoid 3,4-dehydrogenase gene) homolog from marine bacterium strain P99-3 included in the gene cluster for the biosynthesis of myxol (3',4'-didehydro-1',2'-dihydro-beta, psi-carotene-3,1',2'-triol) was functionally identified. The P99-3 CrtD was phylogenetically distant from the other CrtDs. A catalytic feature was its high activity for the monocyclic carotenoid conversion: 1'-HO-torulene (3',4'-didehydro-1',2'-dihydro- beta, psi-caroten-1'-ol) was prominently formed from 1'-HO-gamma-carotene (1',2'-dihydro-beta, psi-caroten-1'-ol) in Escherichia coli with P99-3 CrtD, indicating that this enzyme has been highly adapted to myxol biosynthesis. This unique type of crtD is a valuable tool for obtaining 1'-HO-3',4'-didehydro monocyclic carotenoids in a heterologous carotenoid production system.

MeSH terms

  • Bacteria / enzymology*
  • Carotenoids / chemistry*
  • Catalysis
  • Chromatography, High Pressure Liquid
  • Escherichia coli / metabolism
  • Genetic Complementation Test
  • Models, Chemical
  • Open Reading Frames
  • Oxidoreductases / chemistry*
  • Oxidoreductases / genetics*
  • Phylogeny
  • Substrate Specificity
  • Time Factors
  • Xanthophylls / chemistry*

Substances

  • Xanthophylls
  • myxol
  • Carotenoids
  • Oxidoreductases
  • hydroxyneurosporene desaturase