Abstract
Cloning of the mammalian basic transcription factors serves as a major step in understanding the mechanism of transcription initiation. The 62-kilodalton component (p62) of one of these transcription factors, BTF2 was cloned and overexpressed. A monoclonal antibody to this polypeptide inhibited transcription in vitro. Immunoaffinity experiments demonstrated that the 62-kilodalton component is closely associated with the other polypeptides present in the BTF2 factor. Sequence similarity suggests that BTF2 may be the human counterpart of RNA polymerase II initiation factor b from yeast.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Cloning, Molecular*
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Escherichia coli / genetics
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Gene Expression
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HeLa Cells
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Humans
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Immunoblotting
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Molecular Sequence Data
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Molecular Weight
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Oligonucleotide Probes
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Recombinant Proteins / chemistry
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Sequence Homology, Nucleic Acid
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Transcription Factor TFIIH
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Transcription Factors / chemistry
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Transcription Factors / genetics*
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Transcription Factors, TFII*
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Transcription, Genetic
Substances
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Oligonucleotide Probes
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Recombinant Proteins
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Transcription Factors
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Transcription Factors, TFII
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Transcription Factor TFIIH