Cloning of the 62-kilodalton component of basic transcription factor BTF2

Science. 1992 Sep 4;257(5075):1392-5. doi: 10.1126/science.1529339.

Abstract

Cloning of the mammalian basic transcription factors serves as a major step in understanding the mechanism of transcription initiation. The 62-kilodalton component (p62) of one of these transcription factors, BTF2 was cloned and overexpressed. A monoclonal antibody to this polypeptide inhibited transcription in vitro. Immunoaffinity experiments demonstrated that the 62-kilodalton component is closely associated with the other polypeptides present in the BTF2 factor. Sequence similarity suggests that BTF2 may be the human counterpart of RNA polymerase II initiation factor b from yeast.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cloning, Molecular*
  • Escherichia coli / genetics
  • Gene Expression
  • HeLa Cells
  • Humans
  • Immunoblotting
  • Molecular Sequence Data
  • Molecular Weight
  • Oligonucleotide Probes
  • Recombinant Proteins / chemistry
  • Sequence Homology, Nucleic Acid
  • Transcription Factor TFIIH
  • Transcription Factors / chemistry
  • Transcription Factors / genetics*
  • Transcription Factors, TFII*
  • Transcription, Genetic

Substances

  • Oligonucleotide Probes
  • Recombinant Proteins
  • Transcription Factors
  • Transcription Factors, TFII
  • Transcription Factor TFIIH

Associated data

  • GENBANK/M95809