Abstract
In this study, we investigated the ability of Fusobacterium nucleatum subsp. nucleatum to increase its tissue-invasive potential by acquiring cell-associated human matrix metalloproteinase 9 (MMP-9) activity. Binding of pro-MMP-9 to fusobacteria was demonstrated by enzyme-linked immunosorbent assay. Zymography and a colorimetric assay showed that bound pro-MMP-9 can be converted into a proteolytically active form. The potential contribution of this acquired host activity in tissue invasion was demonstrated using a reconstituted basement membrane (Matrigel).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Basement Membrane / metabolism*
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Basement Membrane / microbiology*
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Collagen / metabolism*
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Collagenases / metabolism*
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Colorimetry
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Drug Combinations
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Enzyme Activation
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Enzyme Precursors / metabolism*
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Enzyme-Linked Immunosorbent Assay
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Fusobacterium nucleatum / metabolism*
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Humans
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Laminin / metabolism*
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Matrix Metalloproteinase 9 / metabolism*
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Protein Binding
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Proteoglycans / metabolism*
Substances
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Drug Combinations
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Enzyme Precursors
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Laminin
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Proteoglycans
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matrigel
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Collagen
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Collagenases
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pro-matrix metalloproteinase 9
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Matrix Metalloproteinase 9