Ultrasensitive electrocatalytic DNA detection at two- and three-dimensional nanoelectrodes

J Am Chem Soc. 2004 Oct 6;126(39):12270-1. doi: 10.1021/ja0458221.

Abstract

Electrochemical DNA detection systems are an attractive approach to the development of multiplexed, high-throughput DNA analysis systems for clinical and research applications. We have engineered a new class of nanoelectrode ensembles (NEEs) that constitute a useful platform for biomolecular electrochemical sensing. High-sensitivity DNA detection was achieved at oligonucleotide-functionalized NEEs using a label-free electrocatalytic assay. Attomole levels of DNA were detected using the NEEs, validating the promise of nanoarchitectures for ultrasensitive biosensing.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biosensing Techniques / methods
  • Catalysis
  • Cations / chemistry
  • DNA / analysis*
  • DNA / chemistry
  • DNA / genetics
  • Electrochemistry / methods
  • Ferric Compounds / chemistry
  • Gold / chemistry
  • Helicobacter pylori / genetics
  • Microelectrodes*
  • Microscopy, Electron, Scanning
  • Nanotechnology / methods*
  • Nucleic Acid Hybridization
  • Oligonucleotides / chemistry
  • Oligonucleotides / genetics
  • RNA, Ribosomal, 23S / analysis
  • RNA, Ribosomal, 23S / genetics
  • Ruthenium / chemistry
  • Sensitivity and Specificity

Substances

  • Cations
  • Ferric Compounds
  • Oligonucleotides
  • RNA, Ribosomal, 23S
  • Gold
  • Ruthenium
  • DNA