Abstract
In the present work, we have used the in silico subtraction methodology to identify six new mouse genes similar to NALP5/MATER, whose ESTs were represented almost exclusively in egg libraries. Five genes were selected for RT-PCR and/or in situ hybridization. These experiments confirmed their oocyte restricted expression. Five of these genes are localized on mouse chromosome 7, as is NALP5/MATER; among them, three are localized in a 300 kb cluster.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens / genetics*
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Antigens / metabolism*
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DNA, Complementary / metabolism
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Egg Proteins / genetics*
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Egg Proteins / metabolism*
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Expressed Sequence Tags
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Gene Library
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In Situ Hybridization
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Mice
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Models, Genetic
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Molecular Sequence Data
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Multigene Family
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Oocytes / metabolism*
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Protein Structure, Tertiary
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
Substances
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Antigens
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DNA, Complementary
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Egg Proteins
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Nalp5 protein, mouse
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RNA, Messenger