In view of the long-term therapy necessary to cure vaginismus and specially persistent cases, we considered using botulin toxin (BT) injections for the treatment of such cases. Eight women (mean age 26.6 +/- 1.2 years) with vaginismus were treated with BT. Another five women with vaginismus, matching the eight patients in age, acted as controls. The patients in the study group were injected with BT (25 IU diluted in 1 ml saline) into each of the two bulbospongiosus muscles. Control patients were injected with saline. Mean follow-up was 10.2 +/- 3.3 months. All the patients injected with BT improved. The couples could achieve satisfactory intromission. No patient was in need of re-injection and there was no recurrence during the follow-up period. Control subjects did not improve with the saline injection into the bulbospongiosus muscle. In conclusion, BT injection effected cure in all of the vaginismus patients with no complications or recurrence. The technique is simple, easy, cost-effective, not time-consuming and can be achieved on an outpatient basis.