Abstract
We have isolated a novel cDNA from the human fetal brain cDNA library with homology to the Mg2+ -dependent serine/threonine protein phosphatase 2C (PP2C) family. The cDNA is 3055 bp in length, and the predicted coding region encodes a 360-amino-acid protein, which shows 99% identity to the PP2C epsilon from rat and mouse. Then we term it human PP2C epsilon gene. The gene is mapped to chromosome 3q26.1 and contains 4 exons. RT-PCR analysis shows that the PP2C epsilon is widely expressed in human tissues and the expression levels in heart, placenta, lung, liver, kidney, and pancreas are relatively high.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Brain / embryology
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Chromosomes, Human, Pair 3
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Cloning, Molecular
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Computational Biology
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DNA, Complementary / metabolism
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Exons
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Gene Library
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Humans
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Introns
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MAP Kinase Signaling System
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Magnesium / chemistry
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Mice
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Models, Genetic
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Molecular Sequence Data
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Phosphoprotein Phosphatases / biosynthesis*
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Phosphoprotein Phosphatases / chemistry
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Phosphoprotein Phosphatases / genetics*
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Protein Phosphatase 2C
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Protein Structure, Tertiary
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RNA, Messenger / metabolism
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Rats
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Tissue Distribution
Substances
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DNA, Complementary
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RNA, Messenger
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PPM1A protein, human
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PPM1B protein, human
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PPM1G protein, human
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Phosphoprotein Phosphatases
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Protein Phosphatase 2C
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Magnesium