Abstract
There are extensive efforts to develop cell-targeting adenoviral vectors for gene therapy wherein endogenous cell-binding ligands are ablated and exogenous ligands are introduced by genetic means. Although current approaches can genetically manipulate the capsid genes of adenoviral vectors, these approaches can be time-consuming and require multiple steps to produce a modified viral genome. We present here the use of the bacteriophage lambda Red recombination system as a valuable tool for the easy and rapid construction of capsid-modified adenoviral genomes.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenoviridae / genetics*
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Bacteriophage lambda / genetics*
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Biotin / genetics
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Biotinylation
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Blotting, Western
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Capsid*
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Cell Line
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Cloning, Molecular
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DNA / metabolism
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Enzyme-Linked Immunosorbent Assay
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Genetic Engineering
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Genetic Therapy
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Genetic Vectors*
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Genome, Viral
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Humans
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Ligands
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Models, Genetic
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Peptide Library
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Peptides / chemistry
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Plasmids / metabolism
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Protein Structure, Tertiary
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Recombination, Genetic*
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Time Factors
Substances
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Ligands
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Peptide Library
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Peptides
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Biotin
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DNA