Abstract
Co-expression of the cobA gene from Propionibacterium freudenreichii and the cbiA, -C, -D, -E, -T, -F, -G, -H, -J, -K, -L, and -P genes from Salmonella enterica serovar typhimurium in Escherichia coli resulted in the production of cobyrinic acid a,c-diamide. A cbiD deletion mutant of this strain produced 1-desmethylcobyrinic acid a,c-diamide, indicating that CbiD is involved in C-1 methylation in the anaerobic pathway to cobalamin. Strains that did not have the cbiP gene also produced 1-desmethylcobyrinic acid a,c-diamide, and strains that had neither cbiP nor cbiA synthesized 1-desmethylcobyrinic acid even in the presence of cbiD, suggesting that CbiA and CbiP are necessary for CbiD activity.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amides / chemistry
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Bacterial Proteins / biosynthesis*
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Bacterial Proteins / genetics*
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Cell Proliferation
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Cobamides / chemistry
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Escherichia coli / metabolism
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Gene Deletion
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Gene Expression Regulation, Bacterial
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Genetic Engineering*
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Methylation
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Models, Chemical
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Models, Molecular
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Plasmids / metabolism
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Porphyrinogens / chemistry
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Propionibacterium / metabolism*
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Salmonella typhimurium / metabolism
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Spectrophotometry
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Ultraviolet Rays
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Vitamin B 12 / analogs & derivatives*
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Vitamin B 12 / biosynthesis
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Vitamin B 12 / chemistry*
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Vitamin B 12 / genetics*
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Vitamin B 12 / metabolism
Substances
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Amides
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Bacterial Proteins
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Cobamides
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Porphyrinogens
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cobyrinic acid
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Vitamin B 12