Identification of a novel secreted glycoprotein of the yeast Saccharomyces cerevisiae stimulated by heat shock

V V Lupashin; S V Kononova; Ratner YeN; A B Tsiomenko; I S Kulaev

doi:10.1002/yea.320080302

Identification of a novel secreted glycoprotein of the yeast Saccharomyces cerevisiae stimulated by heat shock

Yeast. 1992 Mar;8(3):157-69. doi: 10.1002/yea.320080302.

Authors

V V Lupashin¹, S V Kononova, Ratner YeN, A B Tsiomenko, I S Kulaev

Affiliation

¹ Institute of Biochemistry and Physiology of Microorganisms, U.S.S.R.

PMID: 1574924
DOI: 10.1002/yea.320080302

Abstract

A new secreted yeast glycoprotein with an Mr of about 400 kDa (gp400) has been found. The glycoprotein is an O-mannosylated oligomer, whose synthesis and export into culture medium are stimulated by heat shock. Intracellular transport of gp400 is carried out by membrane vesicles distinct from the known constitutive secretory vesicles. Immunological analysis revealed gp400 only in Saccharomyces species.

MeSH terms

Amino Acids / analysis
Cytoplasmic Granules / metabolism
Endoplasmic Reticulum / metabolism
Fungal Proteins / biosynthesis*
Fungal Proteins / chemistry
Fungal Proteins / isolation & purification
Fungal Proteins / metabolism
Glycoproteins / biosynthesis*
Glycoproteins / chemistry
Glycoproteins / isolation & purification
Glycoproteins / metabolism
Glycosylation
Heat-Shock Proteins / biosynthesis*
Heat-Shock Proteins / chemistry
Heat-Shock Proteins / isolation & purification
Heat-Shock Proteins / metabolism
Hot Temperature
Microscopy, Electron
Molecular Weight
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae / ultrastructure

Substances

Amino Acids
Fungal Proteins
Glycoproteins
Heat-Shock Proteins