Abstract
Naturally emerging and deliberately released pathogens demand new detection strategies to allow early recognition and containment. We describe a diagnostic system for rapid, sensitive, multiplex discrimination of microbial gene sequences and report its application for detecting 22 respiratory pathogens in clinical samples.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacterial Infections / diagnosis*
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Bronchoalveolar Lavage Fluid / microbiology
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Bronchoalveolar Lavage Fluid / virology
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Humans
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Nasal Lavage Fluid / microbiology
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Nasal Lavage Fluid / virology
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RNA, Bacterial / chemistry
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RNA, Bacterial / genetics
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RNA, Viral / chemistry
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RNA, Viral / genetics
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Respiratory Tract Infections / diagnosis*
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Respiratory Tract Infections / microbiology
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Respiratory Tract Infections / virology
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Sputum / microbiology
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Sputum / virology
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Virus Diseases / diagnosis*
Substances
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RNA, Bacterial
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RNA, Viral