Functional characterizations of novWUS involved in novobiocin biosynthesis from Streptomyces spheroides

Arch Biochem Biophys. 2005 Apr 1;436(1):161-7. doi: 10.1016/j.abb.2005.01.012.

Abstract

NovW, novU, and novS gene products represent dTDP-4-keto-6-deoxy-D-glucose 3,5 epimarase, C-methyltransferase and dTDP-glucose-4-ketoreductase involved in noviose biosynthetic pathway, respectively. We have expressed three genes to elucidate the functions of NovW, NovU, and NovS in Escherichia coli. NovW and NovU catalyze the formation of dTDP-4-keto-6-deoxy-5-C-methyl-L-lyxo-hexose from dTDP-4-keto-6-deoxy-D-glucose. NovS reduces the product formed from the reaction of NovW with dTDP-4-keto-6-deoxy-D-glucose in the presence of NADH to result in dTDP-l-rhamnose. Furthermore, a pathway for the biosynthesis of noviose is proposed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Carbohydrate Epimerases / classification
  • Carbohydrate Epimerases / metabolism
  • Carbohydrate Epimerases / pharmacology*
  • Catalysis
  • Chromatography, High Pressure Liquid
  • Deoxy Sugars / chemistry
  • Deoxy Sugars / metabolism
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / metabolism
  • Models, Chemical
  • Multigene Family
  • NAD / pharmacology
  • Novobiocin / biosynthesis*
  • Novobiocin / chemistry
  • Nucleoside Diphosphate Sugars / chemistry
  • Nucleoside Diphosphate Sugars / metabolism
  • Streptomyces / metabolism*
  • Thymine Nucleotides / chemistry
  • Thymine Nucleotides / metabolism

Substances

  • Deoxy Sugars
  • Nucleoside Diphosphate Sugars
  • Thymine Nucleotides
  • NAD
  • Novobiocin
  • thymidine diphosphate rhamnose
  • Carbohydrate Epimerases
  • NovW Carbohydrate Epimerase