Isolation of wound healing/regeneration genes using restrictive fragment differential display-PCR in MRL/MPJ and C57BL/6 mice

Godfred Masinde; Xinmin Li; David J Baylink; Bay Nguyen; Subburaman Mohan

doi:10.1016/j.bbrc.2005.02.143

Isolation of wound healing/regeneration genes using restrictive fragment differential display-PCR in MRL/MPJ and C57BL/6 mice

Biochem Biophys Res Commun. 2005 Apr 29;330(1):117-22. doi: 10.1016/j.bbrc.2005.02.143.

Authors

Godfred Masinde¹, Xinmin Li, David J Baylink, Bay Nguyen, Subburaman Mohan

Affiliation

¹ Musculoskeletal Disease Center, JL Pettis VA Medical Center, Loma Linda, CA 92357, USA.

PMID: 15781240
DOI: 10.1016/j.bbrc.2005.02.143

Abstract

Wound healing in mammals can take several weeks to months and the process is always accompanied by scar formation. Wound healing mechanisms that mimic regeneration are not found in most mature mammalian tissues. However, the MRL/MPJ (MRL) mouse has the unique capacity to regenerate ear hole wound completely in less than a month. To identify genes involved in wound healing without a scar, we chose to use restriction fragment differential display-PCR to isolate genes differentially expressed in the MRL (good healer) mouse and the C57BL/6 (poor healer) mouse at different stages of wound healing. We identified 36 genes that were differentially expressed in the regenerating tissue of good and poor healer strains of which several genes are also genetically linked to wound healing and thus are potential candidate genes for scarless wound healing.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Gene Expression Profiling*
Mice
Mice, Inbred C57BL
Polymerase Chain Reaction / methods*
Regeneration / genetics*
Wound Healing / genetics*