This study investigated the physiological and anthropometric characteristics of rugby league players during a competitive season. Sixty-eight rugby league players were allocated into training (n = 52) and nonexercise control (n = 16) groups. The training group participated in 2 field-training sessions per week, with training loads, match loads, and injury rates recorded. Subjects performed measurements of standard anthropometry (height, body mass, and sum of 7 skinfolds), muscular power (vertical jump), speed (10-, 20-, and 40-m sprint), agility (L run), and maximal aerobic power (multistage fitness test) in December (off-season), March (preseason), May (midseason), and August (end season). Increases in maximal aerobic power and muscular power and reductions in skinfold thickness were observed during the early phases of the season when training loads were highest. However, reductions in muscular power and maximal aerobic power and increases in skinfold thickness occurred toward the end of the season, when training loads were lowest and match loads and injury rates were highest. These findings suggest that high overall playing intensity and match loads in end-season matches increase in injury rates in the latter half of the season, and residual fatigue associated with limited recovery between successive matches may compromise the physical development of rugby league players.