Abstract
In this study, we examined changes in expression of calcium/calmodulin-dependent protein kinase IV (CaMKIV) in the mouse brain following chronic morphine treatment. Double immunohistochemical staining showed strong colocalization of CaMKIV with mu-opioid receptors. Chronic treatment with morphine produced an increase in expression of CaMKIV and phosphorylated cAMP response element-binding protein (pCREB) in the CA3 region of the hippocampus, whereas a decrease in CaMKIV and pCREB expression was observed in the caudate putamen. Interestingly, chronic morphine induced a decrease in protein expression of CaMKIV in the basolateral amygdale and the primary somatosensory cortex without any concomitant changes in pCREB. These findings suggest that CaMKIV-dependent signaling may play a role in chronic morphine-induced neuroplasticity in a brain region-specific manner.
Publication types
-
Comparative Study
-
Research Support, N.I.H., Extramural
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Brain / anatomy & histology
-
Brain / drug effects*
-
Brain / enzymology
-
Brain / metabolism
-
Calcium-Calmodulin-Dependent Protein Kinase Type 1
-
Calcium-Calmodulin-Dependent Protein Kinase Type 4
-
Calcium-Calmodulin-Dependent Protein Kinases / metabolism*
-
Cyclic AMP Response Element-Binding Protein / metabolism
-
Gene Expression Regulation / drug effects*
-
Immunohistochemistry / methods
-
Indoles / metabolism
-
Male
-
Mice
-
Morphine / administration & dosage*
-
Narcotics / administration & dosage*
-
Phosphorylation
-
Receptors, Opioid, mu / metabolism
Substances
-
Cyclic AMP Response Element-Binding Protein
-
Indoles
-
Narcotics
-
Receptors, Opioid, mu
-
DAPI
-
Morphine
-
Calcium-Calmodulin-Dependent Protein Kinase Type 1
-
Calcium-Calmodulin-Dependent Protein Kinase Type 4
-
Calcium-Calmodulin-Dependent Protein Kinases
-
Camk1 protein, mouse
-
Camk4 protein, mouse
-
Pnck protein, mouse