Differential chemical diagnosis of primary hyperoxaluria type II. Highly sensitive analysis of optical isomers of glyceric acid by GC/MS as diastereoisomeric derivatives

Yoshito Inoue; Toshihiro Shinka; Morimasa Ohse; Tomiko Kuhara

doi:10.1016/j.jchromb.2005.03.036

Differential chemical diagnosis of primary hyperoxaluria type II. Highly sensitive analysis of optical isomers of glyceric acid by GC/MS as diastereoisomeric derivatives

J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Aug 25;823(1):2-6. doi: 10.1016/j.jchromb.2005.03.036. Epub 2005 Apr 25.

Authors

Yoshito Inoue¹, Toshihiro Shinka, Morimasa Ohse, Tomiko Kuhara

Affiliation

¹ Division of Human Genetics, Medical Research Institute, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Ishikawa 920-0293, Japan. yosh@kanazawa-med.ac.jp

PMID: 16055048
DOI: 10.1016/j.jchromb.2005.03.036

Abstract

We established a separation method for the optical isomers of glyceric acid in urine by modifying the derivatization steps of the procedure used for the screening and diagnosis. The trimethylsilyl derivatization step in the mass screening procedure was replaced by O-acetyl-(+)-2-butylation, and the samples were analyzed under equivalent GC/MS conditions by capillary gas chromatography on a DB-5MS column. This method can be applied to cases that show a high urinary concentration of glyceric acid to obtain a differential diagnosis of primary hyperoxaluria type II and d-glyceric aciduria easily. l-Glyceric acid was also isolated from the urine of healthy controls as one of the main peaks.

MeSH terms

Diagnosis, Differential
Gas Chromatography-Mass Spectrometry / methods*
Glyceric Acids / analysis*
Glyceric Acids / chemistry
Humans
Hyperoxaluria, Primary / diagnosis
Hyperoxaluria, Primary / urine*
Male
Reproducibility of Results
Sensitivity and Specificity
Stereoisomerism

Substances

Glyceric Acids
glyceric acid