Simple fluorescent pyrimidine analogues detect the presence of DNA abasic sites

Nicholas J Greco; Yitzhak Tor

doi:10.1021/ja052000a

Simple fluorescent pyrimidine analogues detect the presence of DNA abasic sites

J Am Chem Soc. 2005 Aug 10;127(31):10784-5. doi: 10.1021/ja052000a.

Authors

Nicholas J Greco¹, Yitzhak Tor

Affiliation

¹ Department of Chemistry and Biochemistry, University of California-San Diego, La Jolla, CA 92093, USA.

PMID: 16076156
DOI: 10.1021/ja052000a

Abstract

A family of simple pyrimidine analogues has been synthesized, and their photophysical properties have been investigated. The most responsive of the family was incorporated in DNA. This isosteric fluorescent DNA analogue monitors denaturation of a DNA duplex via fluorescence and positively detects the presence of abasic sites in DNA duplexes.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, P.H.S.

MeSH terms

DNA / chemistry*
Nucleic Acid Denaturation
Pyrimidines / chemistry*
Spectrometry, Fluorescence

Substances

Pyrimidines
DNA
pyrimidine

Grants and funding

GM 069773/GM/NIGMS NIH HHS/United States