Production of rosmarinic acid and a new rosmarinic acid 3'-O-beta-D-glucoside in suspension cultures of the hornwort Anthoceros agrestis Paton

Planta. 2006 Jan;223(2):369-73. doi: 10.1007/s00425-005-0089-8. Epub 2005 Aug 20.

Abstract

Cell suspension cultures of the hornwort Anthoceros agrestis Paton (Anthocerotaceae) were cultivated and characterized in CB-media containing 2 and 4% sucrose. The suspension cells accumulated rosmarinic acid up to 5.1% of the cell dry weight as well as caffeoyl-4'-hydroxyphenyllactate. Moreover, a more hydrophilic compound was detected which was isolated and identified as rosmarinic acid 3'-O-beta-D-glucoside, a new rosmarinic acid derivative. This new rosmarinic acid derivative was found up to 1.0% of the cell dry weight in suspension cells of A. agrestis.

MeSH terms

  • Anthocerotophyta / metabolism*
  • Carbohydrates / analysis
  • Cell Culture Techniques
  • Cinnamates / chemistry
  • Cinnamates / isolation & purification*
  • Cinnamates / metabolism*
  • Culture Media
  • Depsides
  • Hydrogen-Ion Concentration
  • Hydrolysis
  • Monosaccharides / chemistry
  • Monosaccharides / isolation & purification*
  • Monosaccharides / metabolism
  • Rosmarinic Acid
  • Time Factors

Substances

  • 3'-O-beta-D-glucopyranosyl rosmarinic acid
  • Carbohydrates
  • Cinnamates
  • Culture Media
  • Depsides
  • Monosaccharides