Abstract
We searched for a gene that is up-regulated in response to LPS at a later time point in primary cultured glial cells. Using a Gene Chip Probe Array, we identified stefin A3, which is known as a cysteine protease inhibitor. As assessed by RT-PCR, we observed a time-dependent (2 to 48 h) up-regulation of stefin A3. The results indicate that stefin A3 is involved in infection and inflammation at a later time point.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Cells, Cultured
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Cystatin A
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Cystatin B
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Cystatins / genetics*
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DNA Primers
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Gene Expression Regulation / drug effects*
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Lipopolysaccharides / pharmacology*
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Mice
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Mice, Inbred C57BL
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Neuroglia / cytology
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Neuroglia / physiology*
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Oligonucleotide Array Sequence Analysis
Substances
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Cstb protein, mouse
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Cystatin A
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Cystatins
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DNA Primers
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Lipopolysaccharides
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Stfa3 protein, mouse
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Cystatin B