Abstract
This study compared two published TaqMan PCR assays targeting different regions of the HIV-1 genome for detection of HIV-1 proviral DNA. The gag specific PCR demonstrated a lower sensitivity than the assay targeting the LTR region. The LTR assay is a highly reproducible and specific technique for HIV-1 proviral DNA detection.
Publication types
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Comparative Study
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Evaluation Study
MeSH terms
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DNA, Viral / blood*
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Genes, gag*
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HIV Infections / diagnosis
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HIV Infections / virology
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HIV Long Terminal Repeat
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HIV-1 / genetics
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HIV-1 / isolation & purification*
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Humans
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Polymerase Chain Reaction / methods*
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Proviruses / genetics
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Proviruses / isolation & purification*
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Reproducibility of Results
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Sensitivity and Specificity
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Taq Polymerase
Substances
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DNA, Viral
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Taq Polymerase