Evaluation of two TaqMan PCR assays for the detection of HIV-1 proviral DNA in blood samples

Shannon M Moroney; Loree C Heller; Ray H Widen

doi:10.1016/j.mimet.2005.08.003

Evaluation of two TaqMan PCR assays for the detection of HIV-1 proviral DNA in blood samples

J Microbiol Methods. 2006 May;65(2):350-3. doi: 10.1016/j.mimet.2005.08.003. Epub 2005 Sep 16.

Authors

Shannon M Moroney¹, Loree C Heller, Ray H Widen

Affiliation

¹ Esoteric Testing, Department of Pathology, Tampa General Hospital, P.O. Box 1289, Tampa, FL 33601, USA. smoroney@tgh.org

PMID: 16169104
DOI: 10.1016/j.mimet.2005.08.003

Abstract

This study compared two published TaqMan PCR assays targeting different regions of the HIV-1 genome for detection of HIV-1 proviral DNA. The gag specific PCR demonstrated a lower sensitivity than the assay targeting the LTR region. The LTR assay is a highly reproducible and specific technique for HIV-1 proviral DNA detection.

Publication types

Comparative Study
Evaluation Study

MeSH terms

DNA, Viral / blood*
Genes, gag*
HIV Infections / diagnosis
HIV Infections / virology
HIV Long Terminal Repeat
HIV-1 / genetics
HIV-1 / isolation & purification*
Humans
Polymerase Chain Reaction / methods*
Proviruses / genetics
Proviruses / isolation & purification*
Reproducibility of Results
Sensitivity and Specificity
Taq Polymerase

Substances

DNA, Viral
Taq Polymerase