Isolation, characterization, and sequencing of Candida albicans repetitive element 2

Gene. 1992 Jul 1;116(1):51-7. doi: 10.1016/0378-1119(92)90628-3.

Abstract

A 1059-bp Sau3A fragment, designated Candida albicans repetitive element 2 (CARE-2), was isolated from the genome of the pathogenic yeast, C. albicans. CARE-2 DNA was detected on several C. albicans chromosomes separated by transverse alternating-field electrophoresis. A high degree of interstrain variation in the pattern of hybridizing bands were observed by Southern blot analysis, with a minimum of 10-14 copies of CARE-2 per strain. A low frequency of new CARE-2 polymorphisms was observed over time for three strains grown at 25 degrees C or 37 degrees C. No new CARE-2 polymorphisms were observed from two naturally occurring switch phenotypes. To localize repeated DNA, oligodeoxyribonucleotide probes, each representing a different region of CARE-2, were hybridized to genomic blots. A lower number of copies were observed 5' and 3' to a 600-bp region of CARE-2. Nucleotide (nt) sequence analysis of CARE-2 DNA shows the element is characterized by six perfect direct repeats 6 bp in length and shows no significant DNA similarity with any known nt sequence.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Blotting, Southern
  • Candida albicans / genetics*
  • DNA, Fungal / genetics*
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / genetics
  • Plasmids / genetics
  • Polymorphism, Genetic / genetics
  • Repetitive Sequences, Nucleic Acid / genetics*

Substances

  • DNA, Fungal
  • Oligodeoxyribonucleotides

Associated data

  • GENBANK/M74014
  • GENBANK/M74890
  • GENBANK/M74891
  • GENBANK/M74892
  • GENBANK/M77131
  • GENBANK/M77132
  • GENBANK/M79309
  • GENBANK/M79310
  • GENBANK/S38798
  • GENBANK/S38799