Type II phosphatidylinositol 4-kinase beta associates with TCR-CD3 zeta chain in Jurkat cells

Mol Immunol. 2006 Feb;43(5):454-63. doi: 10.1016/j.molimm.2005.03.009. Epub 2005 Apr 7.

Abstract

Phosphatidylinositol lipid signaling cascades are integral part of TCR-CD3 signaling. The mechanisms by which phosphatidylinositol kinases are coupled to TCR-CD3 complex remain elusive. Here we report an association of type II PtdIns 4-kinase with TCR-CD3 zeta chain upon cross-linking. Mapping studies have revealed that the C-terminal ITAM is critical for docking of the enzyme on the zeta chain. The association is shown to be tyrosyl phosphorylation dependent as mutation of Y-151 and Y-142 on the C-terminal ITAM disrupts interaction of the two proteins. Identification of the associated type II PtdIns 4-kinase revealed that the beta isoform of the enzyme interacts with the zeta chain in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Phosphatidylinositol 4-Kinase / chemistry
  • 1-Phosphatidylinositol 4-Kinase / metabolism*
  • Amino Acid Motifs
  • Antibodies, Monoclonal / immunology
  • Enzyme Activation
  • Humans
  • Immunologic Capping
  • Jurkat Cells
  • Lymphocytes / metabolism
  • Membrane Proteins / chemistry
  • Membrane Proteins / metabolism*
  • Mutagenesis, Site-Directed
  • Phosphorylation
  • Phosphotyrosine / chemistry
  • Protein Interaction Mapping
  • Protein Processing, Post-Translational
  • Receptors, Antigen, T-Cell / chemistry
  • Receptors, Antigen, T-Cell / metabolism*
  • Recombinant Fusion Proteins / metabolism
  • Transfection

Substances

  • Antibodies, Monoclonal
  • Membrane Proteins
  • Receptors, Antigen, T-Cell
  • Recombinant Fusion Proteins
  • antigen T cell receptor, zeta chain
  • Phosphotyrosine
  • 1-Phosphatidylinositol 4-Kinase