Abstract
Four AP2/EREBP genes encoding putative ethylene-responsive element binding factor (ERF)/AP2 domains were cloned from Brassica napus, and these genes could be induced by low temperature, ethylene, drought, high salinity, abscisic acid and jasmonate treatments. These four genes, named BnDREBIII-1 to BnDREBIII-4, were highly homologous and the 37th amino acid was the only difference among their ERF/AP2 domains. BnDREBIII-1 was demonstrated to be able to bind to both dehydration-responsive element and the GCC box and transactivate the expression of downstream genes, while BnDREBIII-4 could bind neither. Further results suggested that Ala37 might play a crucial role in the DNA binding or the stability of the ERF/AP2 domain.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Abscisic Acid
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Adaptation, Physiological / genetics
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Alanine
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Binding Sites
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Brassica napus / genetics*
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Conserved Sequence*
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Cyclopentanes
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DNA, Plant
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DNA-Binding Proteins
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Dehydration
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Ethylenes
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Gene Expression Regulation, Plant*
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Homeodomain Proteins / chemistry
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Homeodomain Proteins / metabolism*
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Nuclear Proteins / chemistry
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Nuclear Proteins / metabolism*
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Oxylipins
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Plant Proteins / chemistry
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Plant Proteins / metabolism*
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Promoter Regions, Genetic
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Response Elements*
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Salts
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Seeds / genetics
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Transcription Factors
Substances
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Cyclopentanes
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DNA, Plant
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DNA-Binding Proteins
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Ethylenes
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Homeodomain Proteins
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Nuclear Proteins
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Oxylipins
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Plant Proteins
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Salts
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Transcription Factors
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jasmonic acid
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Abscisic Acid
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ethylene
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Alanine