Abstract
The gene ENA1 was cloned by its ability to complement the Li+ sensitivity of a low Li(+)-efflux strain. The nucleotide sequence of the cloned DNA fragment showed that there are two almost identical genes in tandem, and predicts that they encode P-ATPases. Disruption of both genes originated a strain defective in Na+ and Li+ effluxes, and sensitive to Na+, to Li+ and to alkaline pH. By transformation with ENA1 the defective effluxes and tolerances were repaired.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biological Transport, Active
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Hydrogen-Ion Concentration
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Lithium / metabolism
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Phosphoproteins / metabolism
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Phosphoproteins / physiology
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Potassium / metabolism
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development
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Sodium-Potassium-Exchanging ATPase / classification
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Sodium-Potassium-Exchanging ATPase / metabolism*
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Sodium-Potassium-Exchanging ATPase / physiology*
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Transformation, Genetic
Substances
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Phosphoproteins
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Lithium
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Sodium-Potassium-Exchanging ATPase
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Potassium