Abstract
Here, the ligand binding, activation, and tissue distribution of the orphan G protein-coupled receptor (GPCR) GPR92 were studied. GPR92 binds and is activated by compounds based on the lysophosphatidic acid (LPA) backbone. The binding of LPA to GPR92 was of high affinity (K(D) = 6.4 +/- 0.9 nM) and led to an increase in both phosphoinositide hydrolysis and cAMP production. GPR92 is atypical in that it has a low sequence homology with the classic LPA(1-3) receptors (21-22%). Expression of GPR92 is mainly found in heart, placenta, spleen, brain, lung, and gut. Notably, GPR92 is highly expressed in the lymphocyte compartment of the gastrointestinal tract. It is the most abundant GPCR activated by LPA found in the small intestinal intraepithelial CD8+ cytotoxic T cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Northern
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Cell Line
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Cyclic AMP / biosynthesis
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DNA, Complementary / biosynthesis
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DNA, Complementary / genetics
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Digestive System / cytology*
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Digestive System / drug effects
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Genes, Reporter / genetics
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Hydrolysis
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Intestinal Mucosa / metabolism
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Intestine, Small / cytology
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Intestine, Small / drug effects
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Intestine, Small / metabolism
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Intestines / cytology
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Intestines / drug effects
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Lymphocytes / drug effects
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Lymphocytes / metabolism*
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Lysophospholipids / metabolism*
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Lysophospholipids / pharmacology*
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Mice
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Phylogeny
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Radioligand Assay
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Receptors, G-Protein-Coupled / drug effects*
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Receptors, Lysophosphatidic Acid / drug effects
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Reverse Transcriptase Polymerase Chain Reaction
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Tissue Distribution
Substances
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DNA, Complementary
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LPAR5 protein, human
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Lysophospholipids
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Receptors, G-Protein-Coupled
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Receptors, Lysophosphatidic Acid
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Cyclic AMP