Abstract
Three unique sorghum flavonoid 3'-hydroxylase (F3'H) cDNAs (SbF3'H1, SbF3'H2 and SbF3'H3) were discovered through bioinformatics analysis. Their encoded proteins showed >60% identity to the Arabidopsis TT7 (F3'H) protein. Overexpression of SbF3'H1 or SbF3'H2 restored the ability of tt7 mutants to produce 3'-hydroxylated flavonoids, establishing their roles as functional F3'H enzymes. In sorghum mesocotyls, SbF3'H1 expression was involved in light-specific anthocyanin accumulation while SbF3'H2 expression was involved in pathogen-specific 3-deoxyanthocyanidin synthesis. No SbF3'H3 expression was detected in all tissues examined. The sorghum mesocotyls represent a good system for investigation of differential regulation of F3'H genes/alleles responding to different external stimuli.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anthocyanins / biosynthesis
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Anthocyanins / chemistry
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Cytochrome P-450 Enzyme System / biosynthesis
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Cytochrome P-450 Enzyme System / genetics*
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DNA, Complementary / analysis
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Gene Expression Regulation, Enzymologic*
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Gene Expression Regulation, Plant*
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Genes, Plant
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Mixed Function Oxygenases / biosynthesis
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Mixed Function Oxygenases / genetics*
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Molecular Sequence Data
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Molecular Structure
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Phytoalexins
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Sesquiterpenes
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Sorghum / genetics*
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Terpenes / chemistry
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Terpenes / metabolism
Substances
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Anthocyanins
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DNA, Complementary
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Sesquiterpenes
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Terpenes
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Cytochrome P-450 Enzyme System
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Mixed Function Oxygenases
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flavonoid 3'-hydroxylase
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Phytoalexins
Associated data
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GENBANK/DQ787855
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GENBANK/DQ787856
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GENBANK/DQ787857