Essentiality of a newly identified carbohydrate-binding module for the function of CelB (BH0603) from the alkaliphilic bacterium Bacillus halodurans

Appl Environ Microbiol. 2006 Oct;72(10):6851-3. doi: 10.1128/AEM.01209-06. Epub 2006 Sep 1.

Abstract

CelB (BH0603) from Bacillus halodurans is a modular glycoside hydrolase with a family 5 catalytic module, an immunoglobulin-like module, and module PfamB of unknown function. The recombinant PfamB module bound to Avicel and was essential for CelB hydrolytic function. We propose that module PfamB be designated a new carbohydrate-binding module.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkalies
  • Bacillus / enzymology*
  • Bacillus / metabolism
  • Carbohydrate Metabolism*
  • Molecular Sequence Data
  • Phosphoenolpyruvate Sugar Phosphotransferase System / metabolism*

Substances

  • Alkalies
  • Phosphoenolpyruvate Sugar Phosphotransferase System
  • cellobiose phosphotransferase B