Enzyme annotation with chemical tools

Ariel G Herman; Brent R Stockwell

doi:10.1016/j.chembiol.2006.10.002

Enzyme annotation with chemical tools

Chem Biol. 2006 Oct;13(10):1013-4. doi: 10.1016/j.chembiol.2006.10.002.

Authors

Ariel G Herman¹, Brent R Stockwell

Affiliation

¹ Department of Biological Sciences, Columbia University, 614 Fairchild Center, 1212 Amsterdam Avenue, MC 2406, New York, New York 10027, USA.

PMID: 17052604
DOI: 10.1016/j.chembiol.2006.10.002

Abstract

Defining the activity of an enzyme in a cell-based system is preferable to in vitro biochemical techniques requiring extensive purification. Chiang et al. use small molecules, RNA interference, and metabolomics to characterize a novel enzyme upregulated in cancer cells.

Publication types

Comment

MeSH terms

Chromatography, High Pressure Liquid / methods
Enzyme Activation / drug effects
Enzyme Inhibitors / chemistry
Enzyme Inhibitors / pharmacology
Enzymes / chemistry
Enzymes / drug effects
Enzymes / metabolism*
Female
Humans
Ketones / chemistry
Ketones / pharmacology
Lysophospholipids / chemistry
Lysophospholipids / metabolism
Ovarian Neoplasms / enzymology
Ovarian Neoplasms / metabolism*
Platelet Activating Factor / metabolism
RNA Interference
Sensitivity and Specificity
Up-Regulation

Substances

11-hexadecenyl trifluoromethyl ketone
Enzyme Inhibitors
Enzymes
Ketones
Lysophospholipids
Platelet Activating Factor