The crucial role of growth factors and cytokines in cutaneous wound healing is well described, but there has been little investigation into their use for skin rejuvenation. A novel skin cream containing a mixture of human growth factors and cytokines was recently marketed for skin rejuvenation. The mixture was obtained through a biotechnology process using cultured human fetal skin cells, which originate from a dedicated cell bank originally established for the development of products in wound healing. The cream significantly reduced periorbital and perioral wrinkles, as well as improved skin texture of the chin after one month of treatment, which confirms the beneficial use of growth factors and cytokines for skin rejuvenation reported in 2 earlier studies. After 60 days of twice-daily application, 83% of the subject showed an improved average wrinkle score in the eye area, while 50% showed an improved average wrinkle score in the mouth area. In order to exclude a placebo effect, the efficacy of this product should be confirmed with a double-blind, randomized, placebo-controlled study. Also, the difference between mixtures of growth factor and cytokines should be further elaborated.