Abstract
We evaluated the effect of morphine on human dendritic cells (DCs). Interestingly, immature DCs were found to express all 3 (mu, kappa, delta) opioid receptors on the cell surface. Chronic morphine treatment (10(-8) to 10(-12) M) during the development of DCs from monocytes augmented LPS-induced upregulation of HLA-DR, CD86, CD80, and CD83 and increased the T cell stimulatory capacity of DCs, which could be inhibited by naloxone, an opioid receptor antagonist. The change in surface phenotype was paralleled by a p38 MAPK-dependent decrease in IL-10 and increase in IL-12 secretion. Our data indicate that morphine exerts an immunostimulatory effect by modulating LPS-induced DC maturation.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology
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Dendritic Cells / drug effects
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Dendritic Cells / immunology*
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Humans
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Imidazoles / pharmacology
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Immunosuppression Therapy
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Interleukin-10 / biosynthesis*
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Interleukin-12 / biosynthesis*
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Lymphocyte Activation / drug effects*
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Lymphocyte Culture Test, Mixed
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Monocytes / immunology*
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Morphine / pharmacology*
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Opioid-Related Disorders / immunology
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Pyridines / pharmacology
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T-Lymphocytes / drug effects
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T-Lymphocytes / immunology*
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p38 Mitogen-Activated Protein Kinases / metabolism
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Imidazoles
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Pyridines
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Interleukin-10
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Interleukin-12
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Morphine
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p38 Mitogen-Activated Protein Kinases
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SB 203580