Antioxidant activity of flavonoids isolated from young green barley leaves toward biological lipid samples

J Agric Food Chem. 2007 Jul 11;55(14):5499-504. doi: 10.1021/jf070543t. Epub 2007 Jun 1.

Abstract

Natural plant flavonoids, saponarin/lutonarin=4.5/1, isolated from young green barley leaves were examined for their antioxidant activity using cod liver oil, omega-3 fatty acids, phospholipids, and blood plasma. The saponarin/lutonarin (S/L) mixture inhibited malonaldehyde (MA) formation from cod liver oil by 76.47+/-0.11% at a level of 1 micromol and 85.88+/-0.12% at a level of 8 micromol. The S/L mixture inhibited MA formation from the omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) by 45.60+/-1.08 and 69.24+/-0.24%, respectively, at a level of 8 micromol. The S/L mixture inhibited MA formation from the phospholipids lecithin I and II by 43.08+/-0.72 and 69.16+/-2.92%, respectively, at a level of 8 micromol. It also inhibited MA formation from blood plasma by 62.20+/-0.11% at a level of 8 micromol. The antioxidant activities obtained from the S/L mixture were comparable to those obtained from alpha-tocopherol and butylated hydroxy toluene (BHT) in all lipids tested.

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Cattle
  • Cod Liver Oil / chemistry
  • Fatty Acids, Omega-3 / chemistry
  • Flavonoids / isolation & purification*
  • Flavonoids / pharmacology*
  • Hordeum / chemistry*
  • Horses
  • Lipid Peroxidation / drug effects*
  • Malondialdehyde / blood
  • Malondialdehyde / chemistry
  • Phosphatidylcholines / chemistry
  • Plant Leaves / chemistry*

Substances

  • Antioxidants
  • Fatty Acids, Omega-3
  • Flavonoids
  • Phosphatidylcholines
  • Malondialdehyde
  • Cod Liver Oil