Recombinant expression and characterization of a reducing-end xylose-releasing exo-oligoxylanase from Bifidobacterium adolescentis

Appl Environ Microbiol. 2007 Aug;73(16):5374-7. doi: 10.1128/AEM.00722-07. Epub 2007 Jun 22.

Abstract

The family 8 glycoside hydrolase (RexA) from Bifidobacterium adolescentis was expressed in Escherichia coli. The recombinant enzyme was characterized as a reducing-end xylose-releasing exo-oligoxylanase. Apart from giving insights into this new class of enzymes, knowledge of the RexA enzyme helps to postulate a mechanism for the B. adolescentis breakdown of prebiotic xylooligosaccharides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Bifidobacterium / enzymology
  • Bifidobacterium / genetics
  • Bifidobacterium / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Glycoside Hydrolases / genetics
  • Glycoside Hydrolases / metabolism*
  • Hydrogen-Ion Concentration
  • Hydrolysis
  • Molecular Sequence Data
  • Phylogeny
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Temperature
  • Xylose / metabolism*

Substances

  • Bacterial Proteins
  • Recombinant Proteins
  • Xylose
  • Glycoside Hydrolases