Abstract
The family 8 glycoside hydrolase (RexA) from Bifidobacterium adolescentis was expressed in Escherichia coli. The recombinant enzyme was characterized as a reducing-end xylose-releasing exo-oligoxylanase. Apart from giving insights into this new class of enzymes, knowledge of the RexA enzyme helps to postulate a mechanism for the B. adolescentis breakdown of prebiotic xylooligosaccharides.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Bifidobacterium / enzymology
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Bifidobacterium / genetics
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Bifidobacterium / metabolism*
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Electrophoresis, Polyacrylamide Gel
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Glycoside Hydrolases / genetics
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Glycoside Hydrolases / metabolism*
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Hydrogen-Ion Concentration
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Hydrolysis
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Molecular Sequence Data
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Phylogeny
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Recombinant Proteins / metabolism
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Sequence Homology, Amino Acid
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Temperature
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Xylose / metabolism*
Substances
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Bacterial Proteins
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Recombinant Proteins
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Xylose
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Glycoside Hydrolases