Suppressive effects of germinated buckwheat on development of fatty liver in mice fed with high-fat diet

Phytomedicine. 2007 Aug;14(7-8):563-7. doi: 10.1016/j.phymed.2007.05.002. Epub 2007 Jun 29.

Abstract

Effects of buckwheat germinated for 48 h in suppressing fatty liver were investigated using an animal study. Concentration of rutin was increased more than 10 times, with production of quercitrin and one newly formed flavonoid during 48 h germination. When an ethanol extract of germinated buckwheat was fed daily to C57BL/6 mice at 100 or 200 mg/kg body wt, along with a high-fat diet, oral administration of germinated buckwheat caused significant reductions in TG and TC levels in the liver after 8 weeks. Oral administration of germinated buckwheat also down-regulated mRNA expressions of PPARgamma and C/EBPalpha in hepatocytes, in a dose-dependent manner. These results suggest that germinated buckwheat has potent anti-fatty liver activities caused partially by suppressing the gene expression of certain adipogenic transcription factors like PPARgamma and C/EBPalpha in hepatocytes.

MeSH terms

  • Animals
  • CCAAT-Enhancer-Binding Protein-alpha / genetics
  • CCAAT-Enhancer-Binding Protein-alpha / metabolism
  • Dietary Fats / adverse effects*
  • Fagopyrum / physiology*
  • Fatty Liver / chemically induced*
  • Fatty Liver / diet therapy*
  • Gene Expression Regulation
  • Germination*
  • Hepatocytes / drug effects
  • Hepatocytes / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • PPAR gamma / genetics
  • PPAR gamma / metabolism
  • Phytotherapy
  • Quercetin / analogs & derivatives
  • Quercetin / chemistry
  • Quercetin / pharmacology
  • Rutin / chemistry
  • Rutin / pharmacology
  • Seeds / physiology

Substances

  • CCAAT-Enhancer-Binding Protein-alpha
  • Dietary Fats
  • PPAR gamma
  • quercitrin
  • Rutin
  • Quercetin