Abstract
In the present study, we have investigated the efficacy of Indian ayurvedic herbal formulation Triphala on monosodium urate crystal-induced inflammation in mice; an experimental model for gouty arthritis and compared it with that of the non-steroidal anti-inflammatory drug, Indomethacin. The anti-arthritic effect of Triphala was evaluated by measuring changes in the paw volume, lysosomal enzyme activities, lipid peroxidation, anti-oxidant status and inflammatory mediator TNF-alpha in control and monosodium urate crystal-induced mice. The levels of beta-glucuronidase and lactate dehydrogenase were also measured in monosodium urate crystal-incubated polymorphonuclear leucocytes (PMNL). Triphala treatment (1 gm/kg/b.w. orally) significantly inhibited the paw volume and the levels of lysosomal enzymes, lipid peroxidation and inflammatory mediator tumour necrosis factor-alpha; however the anti-oxidant status was found to be increased in plasma, liver and spleen of monosodium urate crystal-induced mice when compared to control mice. In addition, beta-glucuronidase and lactate dehydrogenase level were reduced in Triphala (100 microg/ml) treated monosodium urate crystal-incubated polymorphonuclear leucocytes. In conclusion, the results obtained clearly indicated that Triphala exerted a strong anti-inflammatory effect against gouty arthritis.
MeSH terms
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Acetylglucosaminidase / blood
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Acetylglucosaminidase / metabolism
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Acid Phosphatase / blood
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Acid Phosphatase / metabolism
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Administration, Oral
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Animals
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Ankle Injuries / chemically induced
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Ankle Injuries / drug therapy
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Anti-Inflammatory Agents, Non-Steroidal / administration & dosage
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology
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Anti-Inflammatory Agents, Non-Steroidal / standards
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Anti-Inflammatory Agents, Non-Steroidal / therapeutic use
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Antioxidants / metabolism
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Arthritis, Experimental / blood
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Arthritis, Experimental / chemically induced
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Arthritis, Experimental / drug therapy*
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Arthritis, Gouty / blood
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Arthritis, Gouty / chemically induced
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Arthritis, Gouty / drug therapy*
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Dose-Response Relationship, Drug
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Glucuronidase / blood
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Glucuronidase / metabolism
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Indomethacin / administration & dosage
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Indomethacin / pharmacology
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Indomethacin / therapeutic use
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Injections, Intradermal
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L-Lactate Dehydrogenase / metabolism
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Lipid Peroxides / blood
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Lipid Peroxides / metabolism
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Liver / drug effects
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Liver / enzymology
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Lysosomes / drug effects
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Lysosomes / enzymology
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Mice
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Neutrophils / drug effects
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Neutrophils / enzymology
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Neutrophils / metabolism
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Plant Extracts / administration & dosage
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Plant Extracts / pharmacology*
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Plant Extracts / therapeutic use
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Powders
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Spleen / drug effects
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Spleen / enzymology
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Tumor Necrosis Factor-alpha / metabolism
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Uric Acid / administration & dosage
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Uric Acid / toxicity
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beta-Galactosidase / blood
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beta-Galactosidase / metabolism
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Antioxidants
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Lipid Peroxides
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Plant Extracts
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Powders
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Tumor Necrosis Factor-alpha
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triphala
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Uric Acid
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L-Lactate Dehydrogenase
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Acid Phosphatase
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beta-Galactosidase
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Glucuronidase
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Acetylglucosaminidase
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Indomethacin