Abstract
MUC1 (MUC1 in humans and Muc1 in nonhuman species) is a transmembrane mucin-like glycoprotein expressed in epithelial cells lining various mucosal surfaces as well as hematopoietic cells. Recently, we showed that Muc1(-/-) mice exhibited greater inflammatory responses to Pseudomonas aeruginosa or its flagellin compared with their wild-type littermates, and our studies with cultured cells revealed that MUC1/Muc1 suppressed the Toll-like receptor (TLR) 5 signaling pathway, suggesting its anti-inflammatory role. Here we demonstrate that other TLR signaling (TLR2, 3, 4, 7, and 9) is also suppressed by MUC1/Muc1. The results from this study suggest that MUC1/Muc1 may play a crucial role during airway infection and inflammation by various pathogenic bacteria and viruses.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Cell Line
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Cells, Cultured
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Chemokine CXCL1 / analysis
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Chemokine CXCL1 / genetics
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Chemokine CXCL1 / metabolism
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Enzyme-Linked Immunosorbent Assay
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Epithelial Cells / metabolism
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Gene Expression Regulation
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Genes, Reporter
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Humans
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Kidney / cytology
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Luciferases, Renilla / metabolism
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Macrophages, Alveolar / cytology
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Macrophages, Alveolar / metabolism
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Macrophages, Peritoneal / cytology
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Macrophages, Peritoneal / metabolism
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Mice
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Mice, Knockout
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Mucin-1 / genetics*
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Mucin-1 / metabolism*
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Recombinant Proteins / metabolism
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Respiratory Mucosa / cytology
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Respiratory Mucosa / metabolism
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Signal Transduction*
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Statistics as Topic
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Toll-Like Receptors / metabolism*
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Trachea / cytology
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Transfection
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Tumor Necrosis Factor-alpha / analysis
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Tumor Necrosis Factor-alpha / genetics
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Chemokine CXCL1
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Cxcl1 protein, mouse
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Mucin-1
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Recombinant Proteins
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Toll-Like Receptors
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Tumor Necrosis Factor-alpha
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Luciferases, Renilla