Abstract
There have been intensive studies on the differentiation of endothelial progenitor cells (EPCs) into endothelial cells. We investigated the endothelial differentiation of placenta-derived multipotent cells (PDMCs), a population of CD34(-)/CD133(-)/Flk-1(-) cells. PDMCs were cultured in basal media or media containing endothelial growth factors (EGM), including vascular endothelial growth factor (VEGF), for 3 days and then subjected to shear stress of 6 or 12dyn/cm(2) for 24h. Culture of PDMCs in EGM under static conditions resulted in significant increases in VEGF receptor-1 (Flt-1) and receptor-2 (Flk-1) expression. Application of shear stress at 12dyn/cm(2) to these cells led to significant increases in their expression of von Willebrand Factor and platelet-endothelial cell adhesion molecule-1 at both the gene and protein levels. Shear stress at 6dyn/cm(2) had lesser effects. Uptakes of acetylated low-density lipoproteins as well as formation of tube-like structures on Matrigel were significantly increased after subjecting to shear stress of 12dyn/cm(2) for 24h. Our findings suggest that the combined use of endothelial growth factors and high shear stress is synergistic for the endothelial differentiation of PDMCs.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Biomechanical Phenomena
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Cell Adhesion / physiology
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Cell Differentiation / physiology*
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Cell Lineage / genetics
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Cells, Cultured
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Culture Media, Conditioned / pharmacology
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Endothelial Cells / cytology*
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Endothelial Cells / physiology
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Female
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Humans
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Multipotent Stem Cells / cytology*
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Multipotent Stem Cells / physiology
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Placenta / cytology*
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Placenta / physiology
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Platelet Endothelial Cell Adhesion Molecule-1 / biosynthesis
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Platelet Endothelial Cell Adhesion Molecule-1 / physiology
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Pregnancy
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Shear Strength
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Vascular Endothelial Growth Factor A / biosynthesis
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Vascular Endothelial Growth Factor A / physiology
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Vascular Endothelial Growth Factor Receptor-1 / biosynthesis
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Vascular Endothelial Growth Factor Receptor-1 / physiology
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Vascular Endothelial Growth Factor Receptor-2 / biosynthesis
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Vascular Endothelial Growth Factor Receptor-2 / physiology
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von Willebrand Factor / biosynthesis
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von Willebrand Factor / physiology
Substances
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Culture Media, Conditioned
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Platelet Endothelial Cell Adhesion Molecule-1
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VEGFA protein, human
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Vascular Endothelial Growth Factor A
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von Willebrand Factor
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FLT1 protein, human
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Vascular Endothelial Growth Factor Receptor-1
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Vascular Endothelial Growth Factor Receptor-2