Abstract
Roles of Nurr1 and neurogenin 2 (Ngn2) have been shown in midbrain dopamine (DA) neuron development. We present here rat and mouse species-dependent differences of Nurr1 and Ngn2 actions in DA neuron differentiation. Nurr1 exogene expression caused an efficient generation of tyrosine hydroxylase (TH)-positive DA cells from rat neural precursor cells (NPCs). Nurr1-induced TH+ cell yields were low and highly variable depending on the origins of NPCs in mouse cultures. Coexpression of Ngn2 repressed Nurr1-induced generation of TH+ cells in rat cultures. In clear contrast, a robust enhancement in Nurr1-induced DA cell yields was observed in mouse NPCs by Ngn2. These findings imply that DA neurons may develop differently in the midbrains of these two species.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Basic Helix-Loop-Helix Transcription Factors / genetics
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Basic Helix-Loop-Helix Transcription Factors / metabolism*
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Cell Differentiation*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Dopamine / metabolism*
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Gene Expression Regulation
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Humans
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Mice
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism*
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Neurons / cytology*
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Neurons / metabolism
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Nuclear Receptor Subfamily 4, Group A, Member 2
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Rats
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Rats, Sprague-Dawley
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Species Specificity
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Stem Cells / cytology*
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Stem Cells / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Tyrosine 3-Monooxygenase / metabolism
Substances
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Basic Helix-Loop-Helix Transcription Factors
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DNA-Binding Proteins
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NEUROG2 protein, human
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NR4A2 protein, human
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Nerve Tissue Proteins
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Neurog2 protein, mouse
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Nr4a2 protein, mouse
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Nr4a2 protein, rat
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Nuclear Receptor Subfamily 4, Group A, Member 2
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Transcription Factors
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Tyrosine 3-Monooxygenase
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Dopamine